Research Paper Volume 16, Issue 8 pp 7311—7330
EIF3B stabilizes PCNA by counteracting SYVN1-mediated ubiquitination to serve as a promotor in cholangiocarcinoma
Figure 5. EIF3B knockdown inhibited cholangiocarcinoma tumor growth in vivo. (A) Establishment of animal models by implanting EIF3B-knockdown and control RBE cells into female BALB/c nude mice for investigating the role of EIF3B in cholangiocarcinoma tumor growth. (B) The fluorescence intensity in shCtrl and shEIF3B groups was obtained through injecting D-Luciferase. (C–E) Evaluation of tumor growth parameters in the animal models, including tumor volume (C), size (D), and weight (E). (F) Typical images and quantitative data illustrations of EIF3B, PCNA and Ki67 immunohistochemical staining in mice tumor tissues. Scale bar: 50 μm. Magnification times: 200 ×. Results were presented as mean ± SD. * P < 0.05, *** P < 0.001.