Research Paper Volume 16, Issue 8 pp 7217—7248

Figure 8. The loss of the compensatory growth responses of METTL3^Δhep hepatocytes (GPT) to liver injury induced by METTL3^Δhep (GPT). (A–D) The histogram of GO term enrichment analyses based on the GO term of top20 enriched biological process for up-regulated DEGs according to the significance of enrichment (P-value). GO analysis of up-regulated genes in the liver of control mice and METTL3^Δhep mice (JAX) was performed by using RNA-seq data deposited in NCBI GEO under the accession number GSE197800 [16] (A) and GSE176113 [20] (B), respectively, while GO analysis of up-regulated genes in the liver of control mice and METTL3^Δhep mice (GPT) at 1 week (C) and 2 weeks (D) after birth was performed by using RNA-seq data deposited in NCBI GEO under the accession number GSE198512 (C, D). (E, F) IHC of Ki67 in livers from control mice and METTL3^Δhep mice (JAX) at 1 m and 3 m after birth (E), and quantification for Ki67 staining (F). (G, H) IHC of Ki67 in livers from 7-, 14- and 21- day-old control mice and METTL3^Δhep mice (GPT) (G), and quantification for Ki67 staining (H). (I, J) qRT-PCR analysis of the expression of cell cycle-related genes in the liver of METTL3^Δhep mice (JAX) (I) and METTL3^Δhep mice (GPT) (J).