Hepatocyte-specific METTL3 ablation by Alb-iCre mice (GPT), but not by Alb-Cre mice (JAX), resulted in acute liver failure (ALF) and postnatal lethality

Shihao Huang; Yingchun Li; Bingjie Wang; Zhihao Zhou; Yonglong Li; Lingjun Shen; Jinge Cong; Liuxin Han; Xudong Xiang; Jiawei Xia; Danhua He; Zhanlin Zhao; Ying Zhou; Qiwen Li; Guanqi Dai; Hanzhang Shen; Taoyan Lin; Aibing Wu; Junshuang Jia; Dong Xiao; Jing Li; Wentao Zhao; Xiaolin Lin

doi:doi:10.18632/aging.205753

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Research Paper Volume 16, Issue 8 pp 7217—7248

Hepatocyte-specific METTL3 ablation by Alb-iCre mice (GPT), but not by Alb-Cre mice (JAX), resulted in acute liver failure (ALF) and postnatal lethality

Figure 6. Hepatocyte-specific METTL3 homozygous ablation by Alb-Cre mice (JAX) results in apoptosis in mouse hepatocytes. (A) Representative TUNEL staining images of paraffin-embedded liver sections from control mice and METTL3^Δhep mice (JAX) at 1 month or 3 months after birth. Scale bar = 100 μm. (B) Quantification of TUNEL-positive hepatocytes in paraffin-embedded liver sections from control mice and METTL3^Δhep mice (JAX). (C, D) qRT-PCR analysis of the expression of genes related with sphingolipid metabolism in the liver of METTL3^Δhep mice (JAX).