Research Paper Volume 16, Issue 8 pp 7174—7187
Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1
Figure 6. Knockdown of PDZK1 induces glioma cell cycle arrest and cell apoptosis. (A, B) PDZK1 knockdown induced cell cycle arrest in the G0/G1 phase. After transfecting U251 cells with PDZK1 siRNA for 24 h, the cells were collected for cell cycle analysis by PI staining. (B) Quantitation of cell cycle arrest in the G0/G1 phase induced by PDZK1 in (B). (C, D) PDZK1 knockdown induced cell cycle arrest in the G0/G1 phase. After transfecting U87 cells with PDZK1 siRNA for 24 h, the cells were collected for cell cycle analysis by PI staining. (D) Quantitation of cell cycle arrest in the G0/G1 phase induced by PDZK1 in (D). (E) The proportion of apoptotic cells was detected by flow cytometry. PDZK1 knockdown induced U251 and U87 cell apoptosis. (F) The protein expression of cleaved caspase-7, parp1, caspase-3 and GAPDH was detected by Western blotting. PDZK1 knockdown increased the expression of cleaved caspase-7, parp1, and caspase-3.