Research Paper Volume 16, Issue 8 pp 7043—7059

Identification of biomarkers and immune infiltration characterization of lipid metabolism-associated genes in osteoarthritis based on machine learning algorithms

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Figure 8. The changes in intracellular lipid metabolism in OA. (A) Representative images of intracellular lipid droplets stained with BODIPY 493/503. The left side shows normal chondrocytes, and the right side shows OA chondrocytes; Scale bar: 100 μm. (B) Multiplicative changes in the average fluorescence intensity of intracellular lipid droplets stained with BODIPY 493/503. (C) Multiplicative changes in the number of lipid droplets stained with BODIPY 493/503. (D) Measurement of changes in fatty acid oxidation (FAO) in cells from the Normal and OA groups. Dashed lines represent the time points of adding BSA (0.17 mM) or BSA-Palmitate ester (1 mM). *p < 0.05, **p < 0.01, ***p < 0.001.