Research Paper Volume 16, Issue 8 pp 6990—7008

Figure 3. Intervention efficiency of overexpression and interfering RNA lentivirus on ATF6. (A) Representative Western blot image of ATF6 in intervention groups. (B) Representative Western blot image of CTH in intervention groups. (C) Double immunofluorescence analysis of ATF6 (green) and Neuron (red) in brain, arrow indicated ATF6 positive cells. Nuclei were labeled with DAPI (blue). (D) Double immunofluorescence analysis of microglia (yellow) and M2-microglia (red) in brain, arrow indicated M2-microglia positive cells. (E) Western blot analysis quantification of ATF6 in intervention groups. (F) Western blot analysis and quantification of CTH in intervention groups. (G) The relative fluorescent intensity of ATF6 in neuron cells. (H) The percentage of M2-positive microglia was analyzed. Scale bar = 50 μm. The black dots represent individual data in each group. **p < 0.01 and *p < 0.05, ^@@p < 0.01 and ^@p < 0.05, ^##p < 0.01 and ^#p < 0.05, n = 6.