Figure 7. qPCR and Western blot. Relative expression analysis of MMP9 and ITGAM in IS and normal patients. (A, B) represent the relative expression of MMP9 and ITGAM mRNA in control and IS patients by qPCR. (C) q-PCR relative expression of ITGAM in different settings. (D) Western blot of ITGAM and GAPDH in different groups. (E–G) Relative expression of mRNA of TNF-α, IL-1β, and IL-6 when interfering with ITGAM in ischemic stroke. *P < 0.05, **P < 0.01 (n = 4 experiments, Student’s t-test).