Research Paper Volume 16, Issue 7 pp 6521—6536
Garlic oil supplementation blocks inflammatory pyroptosis-related acute lung injury by suppressing the NF-κB/NLRP3 signaling pathway via H2S generation
Figure 3. GO inhibits the activation of NF-κB p65 and NLRP3 inflammasome in LPS-induced ALI. (A) Western blot analysis of phosphorylated NF-κB p65 levels in lung tissue. (B) The grayscale values of the bands in image A were analyzed using ImageJ, and the ratios of p-p65/p65 were calculated. (C) Real-time PCR analysis of mRNA expression levels of the NLRP3/ASC/caspase 1 inflammasome complex in lung tissue. (D) Western blotting analysis of protein expression levels of the NLRP3/ASC/caspase 1 inflammasome complex in lung tissue. (E) A histogram of the grayscale values of the protein bands calculated using ImageJ software (standardized to β-actin). Experiments were performed in triplicate. Student’s t-test, *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviation: ns: no significant difference.