Research Paper Volume 16, Issue 7 pp 6488—6509
Multidimensional data analysis revealed thyroiditis-associated TCF19 SNP rs2073724 as a highly ranked protective variant in thyroid cancer
Figure 4. TCF19[T] reduces its DNA binding ability, thus affecting protein function. (A) CSF3, SRSF6, NCOA5, IL1B, NFE2, MAT2A, TXNIP, GALNT3 mRNA expression levels in cells stably transfected with PCDH, TCF19[C] and TCF19[T] plasmids were measured by qRT-PCR, respectively. (B) CUT&Tag data showed TCF19 could directly bind to the promoter of SRSF6 gene. (C) ChIP-qPCR analysis revealed potential TCF19-binding sites in the SRSF6 promoter region. After SNP mutation, the binding ability was weakened.