Research Paper Volume 16, Issue 7 pp 6455—6477
Machine learning for identifying tumor stemness genes and developing prognostic model in gastric cancer
Figure 15. The effects of CDC25A expression on the proliferation, cell cycle and tumor stemness of gastric cancer cells were verified in vitro. (A) CCK8 assay to detect changes in gastric cancer cell viability after knockdown of CDC25A. (B) Flow cell cycle assay was performed to detect the changes in the percentage of cells in each cycle of gastric cancer cells after knockdown of CDC25A. (C) Cellular immunofluorescence assay to detect changes in MKI67 expression after CDC25A knockdown. (D) Cellular immunofluorescence assay to detect changes in CD44 expression after CDC25A knockdown.