Research Paper Volume 16, Issue 8 pp 6773—6795

Figure 3. HAPSTR1 overexpression enhanced proliferation, migration, and invasion of ovarian cancer cells. (A) Cellular localization was visualized in an immunofluorescence staining assay with microscopic observation at 400x. (B) The mRNA expression level of HAPSTR1 was detected using real-time PCR. (C) Efficiency of HAPSTR1 overexpression was examined using real-time PCR and Western blotting assays. (D, E) Colony formation and CCK-8 assays were used to show improvements in proliferative capacity induced by HAPSTR1 overexpression. (F) The results of the transwell assay showed that HAPSTR1 overexpression enhanced migration and invasion. Original magnification, 200x. (G, H) A wound healing assay was performed to demonstrate increased migration caused by HAPSTR1 overexpression. (I) Flow cytometry was carried out to detect cell apoptosis rates. Each experiment was repeated with three independent replicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001.