Research Paper Volume 16, Issue 7 pp 6417—6444
Pan-cancer analysis of Sushi domain-containing protein 4 (SUSD4) and validated in colorectal cancer
Figure 10. Knockdown of SUSD4 inhibits the cell proliferation and impacts on JAK/STAT pathway. (A) Relative mRNA level of SUSD4 in multiple colorectal cancer cell lines. (B) Validation of siRNA knockdown efficiency in DLD1. (C) Validation of siRNA knockdown efficiency in LOVO. (D) Clonogenic assay and comparison of colony areas. (E) Cell cycle assay. (F) Cell proliferation assays in DLD1. (G) Cell proliferation assays in LOVO. (H) Relative mRNA changes of JAK/STAT pathway key gene between the negative control and knockdown group in DLD1. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.