Research Paper Volume 16, Issue 7 pp 6334—6347
Lactobacillus plantarum 45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation
Figure 5. Detection of expressions of relevant proteins in macrophages with and without knockout of SHP2 via Western blotting. LP45 can exert its anti-oxidative stress effect to activate SHP2, thus modulating inflammatory cytokine expressions to regulate osteoblast and osteoclast differentiation. (A) Western blot was used to detect the expression of SHP2, NOX4 and P47 in SHP2-knockout macrophages. (B) Western blot was used to detect the expressions of NLRP3, IL-1β, p-IRF3, IFN-β and Src in SHP2-knockout macrophages. (C) Expression statistics of SHP2, NOX4 and P47. (D) Expression statistics of NLRP3, IL-1β, p-IRF3, IFN-β and Src. **p < 0.05, Abbreviation: ns: no statistically significant difference.