Research Paper Volume 16, Issue 7 pp 6334—6347
Lactobacillus plantarum 45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation
Figure 3. Detection of expressions of relevant proteins in osteoblasts via immunofluorescence and Western blotting. LP45 promotes osteoblast differentiation by regulating histone proteases in macrophages and secreting exosomes to osteoblasts. (A) Detection of RANKL expression in osteoblasts via immunofluorescence. (B) RANKL expression in osteoblasts of each group. (C) Detection of expressions of relevant proteins in osteoblasts via Western blotting. (D) Expression levels of relevant proteins in osteoblasts. (E) CCK8 experimental results. **p < 0.05, Abbreviation: ns: no statistically significant difference.