Research Paper Volume 16, Issue 8 pp 6757—6772
USP15 facilitates the progression of bladder cancer by amplifying the activation of the NF-κB signaling pathway
Figure 7. USP15 activates NF-κB signaling pathway by upregulating BRCC3 protein expression. (A) Western blotting was used to detect the expression of USP15 and BRCC3 proteins in J82 cells stably transfected with control shNC RNA or USP15 shRNA. (B) Relative mRNA expression of BRCC3 in normal and bladder cancer tissues. (C, D) Scatter plot of correlation between BRCC3 expression and USP15 expression. (E) Western blotting was used to detect the expression of USP15, BRCC3, P65 and p-P65 proteins in J82 cells stably transfected with USP15 shRNA or Flag-BRCC3 or both. (F) The upregulation of BRCC3 significantly saved the proliferation of J82-shUSP15 cells (**p < 0.01). The upregulation of BRCC3 significantly saved the migration and invasion of J82-shUSP15 cells (**p < 0.01).