Research Paper Volume 16, Issue 7 pp 6163—6187
KIF3C: an emerging biomarker with prognostic and immune implications across pan-cancer types and its experiment validation in gastric cancer
Figure 10. KIF3C knockdown suppresses the malignant phenotype of GC cells. (A) Knockdown efficiency of KIF3C in two Gastric cancer cell lines HGC-27 and AGS, including the si-NC and si-KIF3C groups. (B) CCK-8 assays were applied to detect the effect of KIF3C knockdown on the proliferation of HGC-27 and AGS cell lines. (C) Images of colony formation assay results after KIF3C knockdown in the HGC-27 and AGS cell lines. (D) Representative statistical analysis of the colony formation assay results. (E) DNA replication activity was assessed by an EdU-staining assay (green indicates the EdU-incorporated cells, blue indicates nuclei). GAPDH was used as an internal control. (F) Representative statistical analysis of the EdU assays results. (G) Wound-healing assays employed to detect the migration ability of KIF3C knockdown cells, including HGC-27 and AGS cells. (H) Representative statistical analysis of the wound-healing assays results. *p < 0.05; **p < 0.01; ***p < 0.001.