Figure 8. Overexpression of MLCK abolished the beneficial effects of Lifibrate in ZO-1 and endothelial permeability. Cells were transducted with lentiviral-MLCK, followed by exposure to OGD/R with or without Lifibrate (6 μM). (A) Successful overexpression of MLCK was detected by Western blot analysis; (B) mRNA of ZO-1; (C) Endothelial permeability; (D) TEER on the endothelial monolayer (n=6, **, P<0.01 vs. vehicle group; ##, P<0.01 vs. OGD/R group; ^^, P<0.01 vs. OGD/R+ Lifibrate group).