Research Paper Volume 16, Issue 7 pp 6118—6134
A novel risk signature based on liquid-liquid phase separation-related genes reveals prognostic and tumour microenvironmental features in clear cell renal cell carcinoma
Figure 1. LLPS differential gene screening and risk signature establishment and assessment. (A) Heatmap demonstrating the expression of LLPS-related genes in the TCGA-KIRC cohort; (B) Volcano plot demonstrating the LLPS-based expression in the TCGA-KIRC cohort, containing 60 down-regulated genes and 114 up-regulated genes; (C, D) LASSO Cox regression analysis was conducted to screen the key genes; (E) Kaplan-Meier analysis of survival differences between high-risk and low-risk groups in the TCGA-KIRC cohort; (F) ROC curves demonstrating the accuracy of risk-signature judgement of LLPS-related gene constructs for ccRCC patients with 1-, 3- and 5-year prognosis; (G) Kaplan-Meier analysis in the E-MTAB-1980 cohort to validate the survival difference between the high-risk and low-risk groups distinguished by this risk signature; (H) ROC curve to validate the ability of this signature to judge prognosis in the E-MTAB-1980 cohort. (*p < 0.05, **p < 0.01, ***p < 0.001).