Research Paper Volume 16, Issue 7 pp 5916—5928
Downregulating miRNA-199a-5p exacerbates fluorouracil-induced cardiotoxicity by activating the ATF6 signaling pathway
Figure 5. miRNA-199a-5p attenuates 5-FU induced toxicity via ATF6 in vitro. (A–C) The mimic of miRNA-199a-5p and Ad-ATF6 were co-transfected into cardiomyocytes, and the miRNA-199a-5p mimic could not attenuate 5-FU induced toxicity by cell viability, SOD activity, GSH/GSSG ratio and LDH level; (D) Western blot analysis indicates ATF6 was downregulated along with downregulated expression of GRP78, CHOP and PERK in cardiomyocyte.