Research Paper Volume 16, Issue 7 pp 5916—5928
Downregulating miRNA-199a-5p exacerbates fluorouracil-induced cardiotoxicity by activating the ATF6 signaling pathway
Figure 2. 5-FU treatment leads to down-regulated miRNA-199a-5p in cardiomyocytes and myocardial tissue. (A) miRNA-199a-5p was down-regulated after primary cardiomyocytes were treated with 5-FU; (B) down-regulated miRNA-199a-5p could be observed in 5-FU-treated rats when compared with rats receiving vehicle treatment; (C) the miRNA-199a-5p mimic was introduced to primary cardiomyocytes, RT-PCR was performed to measure the transfection efficacy; (D–G) the upregulation of miRNA-199a-5p improved the cell viability caused by 5-FU; increased GSH/GSSG ratio and SOD activity, decreased LDH levels compared with miRNA-199a-5p NC.