Research Paper Volume 16, Issue 6 pp 5651—5675
Single cell RNA-seq identifies a FOS/JUN-related monocyte signature associated with clinical response of heart failure patients with mesenchymal stem cell therapy
Figure 6. NMF subtype analyses uncovers that therapy-response monocyte signatures were capable of partitioning post-MI HF patients into three groups. (A) Consensus clustering for the GSE59867 data based on the hub genes, which contained PBMC samples from stable coronary diseases to heart failure. (B) Heatmap shows the differences among the three patient groups. (C) Heat map displays the infiltration levels of immune cells among the three patient groups. CIBERSOFT algorithm in IOBR packages was adopted. (D) Boxplot shows the signature score of therapy-response monocytes in bulk RNA-seq levels between control and heart failure groups. (E) Correlation analysis of the hub genes and monocytes in bulk RNA-seq levels. The monocytes were highlighted. (F) Infiltration levels of deconvoluted immune cells in the NMF groups. (G) Box plot reveals the signature score of therapy-response monocytes among the three patient groups. (H) Distribution of different clinical information in the NMF groups. NMF: Nonnegative Matrix Factorization.