Research Paper Volume 16, Issue 6 pp 5634—5650

Figure 9. SLC2A1 and ACSL4 were overexpressed in AKI and promote HK-2 cell apoptosis. (A) HE and immunohistochemistry of SLC2A1, GPX4 and TEM in renal ischemia-reperfusion injury model; (B) The protein level of SLC2A1 and ACSL4 in renal ischemia-reperfusion injury model and normal control model was verified by Western blot; (C) Relative quantification of SLC2A1 and ASCL4 in B; (D) The protein level of ACSL4 and SLC2A1 in HR induced AKI model and was verified by Western blot; (E) Relative quantification of these proteins in D; (F) The lipid ROS level was verified in HR induced AKI model and normal control model; (G) The MDA level was verified in HR induced AKI model and normal control model; (H, I) The apoptosis rate was verified in HR induced AKI model and normal control model. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. Abbreviations: MDA: Malondialdehyde; ROS: Reactive Oxygen Species.