Figure 1. Single-cell clusters were identified based on scRNA data from AKI patients. (A) The tSNE diagram shows 9 clusters and the expression of iron-related marker genes associated with iron-induced cell death. (B) The tSNE diagram illustrates the distribution of 8 cell subsets after clustering. (C) The heat map shows the expression levels of the top 5 marker genes in each subpopulation. (D) The tSNE map displays the distribution of 8 marker genes. (E) The volcano map demonstrates the expression patterns of the first 5 marker genes within each subpopulation. (F) Subgroup proportions and cell counts are depicted in 4 sample groups. (G) The distribution of gene expression is compared between the two groups.