Research Paper Volume 16, Issue 6 pp 5567—5580
CRIF1 attenuates doxorubicin-mediated mitochondrial dysfunction and myocardial senescence via regulating PXDN
Figure 7. CRIF1 overexpression inhibited PXDN expression in DOX-induced myocardial tissues and AC16 cells. (A) Western blot detected the expression of PXDN in myocardial tissues. (B) Western blot detected the expression of PXDN in AC16 cells. (C) CO-IP experiment was used to detect the relationship between CRIF1 and PXDN. (D) 293T were transfected with flag-CRIF1 and/or myc-PXDN, and CO-IP assay followed by western blot was performed to examine the myc-tagged protein or flag-tagged protein. ***P<0.001 vs Control; ###P<0.001 vs DOX+Ov-NC.