Research Paper Volume 16, Issue 6 pp 5526—5544
Cinobufotalin regulates the USP36/c-Myc axis to suppress malignant phenotypes of colon cancer cells in vitro and in vivo
Figure 4. USP36 promotes c-Myc expression via its deubiquitinating role. (A) Co-IP assays of USP36 in HCT116 cells. (B) Co-IP assays of c-Myc in HCT116 cells. (C) Co-IP assays of Flag in HCT116 cells transfected with Flag-USP36 or HA-c-Myc or their combination. (D) Co-IP assays of HA in HCT116 cells transfected with Flag-USP36 or HA-c-Myc or their combination. (E) In vitro ubiquitination assay for HCT116 cells with the transfection of HA-c-Myc combined with or without Flag-USP36. (F) CHX half-life assay. *p < 0.05 and **p < 0.01.