Research Paper Volume 16, Issue 7 pp 5866—5886
NXPH4 can be used as a biomarker for pan-cancer and promotes colon cancer progression
Figure 9. The expression of NXPH4 in colon cancer tissues was verified and knocked down in cell lines. (A, B) Western blot was used to verify the expression of NXPH4 at the protein level in colon cancer and para-cancer tissues. (C) NXPH4 was knocked down in HT-29 cell line and verified by Western blot. (D) NXPH4 was knocked down in HT-29 cell line and verified by qPCR. (E) NXPH4 was knocked down in HCT-116 cell line and verified by Western blot. (F) NXPH4 was knocked down in HCT-116 cell line and verified by qPCR. (G) CCK8 assay of control HT-29 cell line and HT-29 cell line with NXPH4 knockdown. (H) CCK8 assay for control HCT-116 cell line and HCT-116 cell line with NXPH4 knockdown.