Research Paper Volume 16, Issue 6 pp 5224—5248

Figure 4. Functional annotation of core genes in the biomarker panel based on consensus clustering in CD. (A) Consensus score matrix of all samples when the number of clusters (k) is 2. (B) CDF curves of the consistency matrix for each k-value. (C) Differential analysis of the five core genes in the two subtypes (cluster 1 and cluster 2) obtained by consensus clustering. (D) Unsupervised PCA plots of samples in two new groups (high-risk and low-risk). (E) Volcano plots of DEGs in the high-risk and low-risk groups. (F) Gene ontology biological process and (G) KEGG enrichment analysis of DEGs between the high-risk and low-risk groups. GSEA of (H) TNF, (I) IL-17, and (J) Toll-like receptor signaling pathways. Abbreviations: CDF: cumulative distribution function; PCA: principal component analysis; KEGG: Kyoto Encyclopedia of Genes and Genomes; DEG: differentially expressed gene; GSEA: gene set enrichment analysis; TNF: tumor necrosis factor. ^****P < 0.0001.