Research Paper Volume 16, Issue 5 pp 4904—4919

Figure 6. Stable expression of miR-18a-5p improved self-renewal of UCMSCs. (A) Western blot assay of Oct4, Nanog and P16 in miR-18a-5p and control lentivirus vector transduced UCMSCs. (B, C) Competitive growth assay of miR-18a-5p and control transduced UCMSCs. GFP ratios were measured by flow cytometry before and after two passaging cultures. (D) GFP ratio of UCMSCs in competitive growth assay before and after culture. The efficiency of osteogenic differentiation (E) was increased in UCMSCs stably expressing miR-18a-5p (F), and similarly, the potential for adipogenic differentiation (G) was enhanced in the group with stable miR-18a-5p transduction. Osteogenic efficiency was evaluated by ALP staining; adipogenic efficiency was evaluated by oil red O staining. The results were quantized in Figure F and Figure H. ^*p < 0.05; ^**p < 0.05.