Research Paper Volume 16, Issue 6 pp 5184—5206

Figure 5. BMSCs-derived exosomes enhance macrophage/microglia autophagy and promote motor function recovery after SCI by delivering miR-21a-5p. (A) Transfection efficiency of miR-21a-5p overexpression and knockdown in BMSCs; (B) The relative expression of miR-21a-5p in BMSCs-derived exosomes in indicated groups; (C) The relative expression of miR-21a-5p in BV2 cells administered with miR-NC^OE-Exos, miR-21a-5p^OE-Exos, miR-NC^KD-Exos, and miR-21a-5p^KD-Exos; (D) BMS was used to functionally grade mice in different groups on the 28th day after injury; (E) MEP analysis was used as an electrophysiological assessment after SCI. (F) Quantification of MEP amplitudes and latencies in mice (n = 6); (G) Representative immunostaining images of IBA1 and Beclin1 in mice of different groups on the 7th day after injury; (H) Quantification of fluorescence intensity; (I) Representative immunostaining images of IBA1 and p62 in mice of different groups on the 7th day after injury; (J) Quantification of fluorescence intensity; (K–O) Western blot detection and quantitative analysis of Beclin1, ATG5, ATG7, LC3-II (^*p < 0.05, ^**p < 0.01,^***p < 0.001).