Research Paper Volume 16, Issue 5 pp 4832—4840
Chlorogenic acid prevents ovariectomized-induced bone loss by facilitating osteoblast functions and suppressing osteoclast formation
Figure 1. Chlorogenic acid increases proliferation and differentiation in osteoblasts. (A) MG-63 cells were treated indicated concentration of chlorogenic acid for 2 days, the cell viability was examined by MTT assay. (B) MG-63 cells were treated indicated concentration of chlorogenic acid for 2 days, the ALP activity was examined by ALP activity kit. MG-63 cells were incubated with osteoblast differentiation medium (vitamin C (50 μg/mL) and β-glycerophosphate (10 mM)) and chlorogenic acid for 3 and 21 days, the bone mineralization was examined by ALP (C) and von Kossa (D) staining. *p < 0.05 vs. control group.