Research Paper Volume 16, Issue 5 pp 4789—4810
SORBS1 inhibits epithelial to mesenchymal transition (EMT) of breast cancer cells by regulating PI3K/AKT signaling and macrophage phenotypic polarization
Figure 7. SORBS1 induces M1-type polarization in THP-1 cells. After transfection of induced differentiated THP-1 cells with SORBS1 overexpression plasmid and siRNA for 48 h. (A) Expression levels of CD68, CD86, CD163, and CD206 mRNAs were detected by QPCR assay. (B) Changes in the proportions of CD68+, CD86+, CD206+ and CD163+ were detected by flow assay in THP-1 cells after different treatments. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).