Figure 4. β-sitosterol attenuates fibrosis markers and serum inflammatory markers in CCL4 model mice. (A) Differential protein expression of the liver fibrosis markers COL1A1, α-SMA, Desmin and Vimentin by immunohistochemistry. (B–E) Relative expression area of fibrosis markers, showing representative results from 6 independent replicate trials (n = 6). Compared to CCL4; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. (F–I) Expression level of LPS, TNF-α, IL-1and IL-6 proteins in the serum of CCL4-induced mice (n=6) compared with control mice, β-sitosterol low-dose and β-sitosterol high-dose (Corn, n=6, β-sitosterol low-dose, n=6, β-sitosterol high-dose, n=6) measured by ELISA assay. Compared to CCL4; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.