Research Paper Volume 16, Issue 5 pp 4759—4777
Network pharmacology and transcriptomic profiling elucidate the therapeutic effects of Ranunculus ternatus Thunb on liver fibrosis via MK3-NF-κB inhibition
Figure 3. Animal experiment flow chart and evaluation of β-sitosterol for the alleviation of CCL4 liver fibrosis in mice. (A) Experimental schedule. (B) Liver sections stained with hematoxylin and eosin (H&E), sirius red, trichrome masson and collagen Iα are shown and observed under a light microscope. (C, D) β-sitosterol alleviates the CCl4 induced elevation in serum AST and ALT in mice. (E) β-sitosterol alleviates weight loss in CCL4 mice. Representative results of at least six independent experiments (biological replicates) are shown in all panels. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.