Research Paper Volume 16, Issue 5 pp 4116—4137

Figure 5. Effect of PR55α on the cell cycle response of normal and cancer cells to IR. Log-phase growing HPNE (Control- or PR55α-transduced) and CD18/HPAF cells (Control-shRNA or PR55α-shRNA transduced) were incubated in media containing 1 μg/ml Dox for 3 days and 2 μg/ml Dox for 5 days, respectively, to manipulate PR55α expression. The cells were exposed to IR at indicated doses, incubated for 24 hours, and then stained with propidium iodide (PI) and analyzed for DNA content using FACS. (A) Representative FACS analyses are shown. The location and percent of cells in the G1, S, and G2/M phases of the cell cycle are indicated by arrows. (B) Bar graphs depicting the percent cells in the G1, S, and G2/M phases of the cell cycle. Each bar represents the mean ± S.D. of two separate experiments, each done in duplicates.