Research Paper Volume 16, Issue 5 pp 4631—4653
miR-26a-5p inhibits the proliferation of psoriasis-like keratinocytes in vitro and in vivo by dual interference with the CDC6/CCNE1 axis
Figure 2. Verification of cross-species conservation of the miR-26a-5/CDC6/CCNE1 axis. (A) Multiple sequence alignment of orthologous miRNAs in the miR-26 family. (B) Comparison of the minimum free energy of miR-26a-5p at the 3′UTR binding sites of target genes between humans (blue columns) and mice (orange columns). (C, D) Dual-luciferase reporter assay of target genes of miR-26a-5p and statistical analysis of the relative fluorescence intensity of each transfection group. The data are presented as the mean ± SD (n = 3). *p < 0.05. ***p < 0.001.