Research Paper Volume 16, Issue 5 pp 4631—4653
miR-26a-5p inhibits the proliferation of psoriasis-like keratinocytes in vitro and in vivo by dual interference with the CDC6/CCNE1 axis
Figure 1. In silico identification of the miR-26a-5p/CCNE1/CDC6 axis in psoriasis (A) GSEA bar chart showing the top 10 enhanced and top 10 inhibited KEGG pathways for DEGs; the color scale represents the FDR, and the length of the bar represents the normalized enrichment score (NES) (positively correlated, blue >0; negatively correlated, orange <0). (B) The interaction network of 10 downregulated miRNAs and target genes involved in the cell cycle and the IL17 pathway. Let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, and let-7g-3p are uniformly represented by let-7 because they all share the same target genes. (C) Multifactor correlation estimation between miR-26a-5p and target genes. (D) Target gene expression in HaCaT and HEKs cells. Orange columns: cells induced with TNF-α without miRNA transfection; blue columns: cells induced with TNF-α and transfected with miR-26a-5p. The data are presented as the mean ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.