Research Paper Volume 16, Issue 5 pp 4591—4608

Figure 7. GBA3 interacted with EP300 to promote CPT2 transcription. (A) Relative mRNA expression, P-value was calculated by 2-tailed Student’s t test (***, P < 0.001; ns, not significant). (B) Immunoblotting detection of CPT2. P-value was calculated by 2-tailed Student’s t test (*, P < 0.05). (C) Heat plot showed the normalized expression profile of transcription factors of CPT2 in GSE160016. (D) Relative mRNA expression of 8 transcription factors, P-value was calculated by 2-tailed Student’s t test (*, P < 0.05; **, P < 0.01; ns, not significant). (E) Immunoblotting detection of EP300. P-value was calculated by 2-tailed Student’s t test (*, P < 0.05). (F) Coimmunoprecipitation of GBA3 with endogenous EP300. (G) Representative images of immunofluorescence staining of GBA3 and EP300. Nuclei were counterstained blue with DAPI (Scale bars, 5 μm). (H) Knockdown of EP300 in cells overexpressing GBA3. (I) CPT2 expression was confirmed through PCR and Western blot. P-value was calculated by 2-tailed Student’s t test (***, P < 0.001). (J) Respiratory rates expressed as oxygen consumption rate (OCR) were measured using Seahorse metabolic analyzer. P-value was calculated by 2-tailed Student’s t test (***, P < 0.001).