Research Paper Volume 16, Issue 6 pp 5091—5107

Figure 2. The effects of depleting GSG2 on thyroid cancer in vitro and in vivo. (A) CCK8 assay demonstrating a significant reduction in proliferation of B-CPAP and TPC-1 cells upon shGSG2-1 and shGSG2-2 transfection. (B) After shGSG2-1 and shGSG2-2 transfection, Colony formation assay illustrated a significant decrease in colony formation abilities of both B-CPAP and TPC-1 cells. (C) The effects of GSG2 knockdown on migration potential were assessed through Transwell assay. (D) The changes in B-CPAP and TPC-1 cell apoptosis following GSG2 depletion were analyzed through flow cytometry analysis. (E, F) Xenograft tumor models were established via subcutaneously injecting shCtrl/shGSG2-transfected TPC-1 cells, and tumor volume (E) and weight (F) were measured. Tumor volume=π/6×L×W×W. (G) Tumors were collected and photographed. (H) Immunohistochemical staining of Ki67 in mice tissues from the shCtrl and shGSG2 groups. Results were presented as mean ± SD. ** P < 0.01, ***P < 0.001.