Research Paper Volume 16, Issue 5 pp 4299—4326

Figure 9. ST3GAL1 was directly regulated by tRFdb-3013a and tRFdb-3013b in colon adenocarcinomas. (A) ST3GAL1 was highly expressed in colon adenocarcinomas compared to non-tumor controls of TCGA-COAD and GSE39582 data (log₂TPM, both P <0.01), COAD: colon adenocarcinomas, CRC: colorectal cancers. (B-left) The luciferase reporter assay of colon adenocarcinoma cell co-transfected with pmiR-RB-ST3GAL1 wild-type or mutant report vector, together with tRFdb-3013a/b or NC mimic. (C) Expression levels of ST3GAL1 mRNA (B-right) and protein in colon adenocarcinomas cells transfected with tRFdb-3013a/b mimic or control. ** P < 0.01, * P < 0.05. The “tRF-3013a/b” means as tRFdb-3013a/b, “NC” means as negative control.