Research Paper Volume 16, Issue 5 pp 4250—4269
Lei’s formula attenuates osteoarthritis mediated by suppression of chondrocyte senescence via the mTOR axis: in vitro and in vivo experiments
Figure 8. LSF ameliorates the progression of OA and inhibits mTOR expression in the mice DMM model. (A) Immunohisto- chemical typical images of pathological sections of mice knee joints from different experimental groups (SHAM, DMM, DMM+LSF-L (0.08 g/kg), DMM+LSF-M (0.17 g/kg), and DMM+LSF-H (0.35 g/kg)), which were examined for the detection of Col2, ACAN, Adamts5, MMP13, Col10, P16, P21 and mTOR expression in mice cartilage. (Scale bar. 50 μm). (B–I) Quantitative results of immunohistochemical staining in 5 groups of mice. All data represent mean ± SD. compared to the SHAM group; ****P < 0.0001; compared to the DMM group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.