Research Paper Volume 16, Issue 5 pp 4250—4269

Figure 6. mTOR-SiRNA contributes to LSF suppression of H₂O₂-stimulated chondrocyte senescence and promotion of anabolism. (A) Westen blotting for protein expression of Col2, Adamts5, P16 and mTOR in lentivirus-transfected chondrocytes stimulated by H2O2 (200 μmol/L). (B–E) Quantitative results of Western blotting of the 4 groups of proteins. (F–M) Expression of Col2, Sox9, MMP13, Adamts5, P16, P21, mTOR, and S6 in lentivirus-transfected chondrocytes stimulated with H2O2 (200 μmol/L) was detected by qRT-PCR. (N) Cellular senescence was assessed by SA-β-gal staining. Compared to Con-SiRNA control group, ****P < 0.0001; compared to the H₂O₂-stimulated group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; compared to the mTOR-SiRNA group, *P < 0.05, **P < 0.01, ***P<0.001, ****P < 0.0001.