Research Paper Volume 16, Issue 5 pp 4250—4269

Figure 5. Activation of mTOR exacerbates H₂O₂-stimulated chondrocyte senescence and anabolic catabolic disorders that can be reversed by LSF. (A) Western blotting for protein expression of Col2, Adamts5, P16 and mTOR in chondrocytes processed with H₂O₂ (200 μmol/L) and MHY1485 (10 μmol/L). (B–E) Quantitative results of Western blotting of the 5 groups of proteins. (F–M) Expression of Col2, Sox9, MMP13, Adamts5, P16, P21, mTOR, and S6 in chondrocytes processed with H₂O₂ (200 μmol/L) and MHY1485 (10 μmol/L) was detected by qRT-PCR. (N) Cellular senescence was assessed by SA-β-gal staining. Compared to the control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; compared to the H₂O₂-stimulated group, *P <0.05, *P < 0.01, ***P < 0.001, ****P<0.0001; compared to the H₂O₂-stimulated combined MHY1485 group, *P < 0.05, **P < 0.01, ***P <0.001, ****P < 0.0001.