Research Paper Volume 16, Issue 5 pp 4250—4269
Lei’s formula attenuates osteoarthritis mediated by suppression of chondrocyte senescence via the mTOR axis: in vitro and in vivo experiments
Figure 3. LSF regulates chondrocyte anabolic-catabolic homeostasis and inhibits chondrocyte senescence. (A–D) Western blotting assessed the expression of Col2, Adamts5 and P16 proteins in chondrocytes. (E–J) qRT-PCR was performed to detect the expression of Col2, Sox9, MMP13, Adamts5, P16 and P21 in chondrocytes. (K) Cellular senescence was assessed by SA-β-gal staining. All data represent mean ± SD. Compared to the control group, **P < 0.01, ***P < 0.001, ****P < 0.0001; compared to the H2O2-stimulated group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; compared to the same concentration of blank serum group, *P < 0.05, **P < 0.01, ****P < 0.0001.