Research Paper Volume 16, Issue 4 pp 3068—3087

Figure 1. (A) Schematic representation of experimental design of senescence induction in the colon fibroblasts. Senescence was induced using hydrogen peroxide (H₂O₂, 400 μM), doxorubicin (doxo, 250 nM) or bleomycin (bleo, 10 ng/mL). Non-senescent proliferating (NS) cells were used as a control. (B) Representative SA- β -gal assay results at pH6 to confirm senescence induction in >70% of the cells. (C) Quantification of SA-β-gal positive senescent cells. (data are Mean ± SEM ^*p < 0.01 versus NS).