Research Paper Volume 16, Issue 5 pp 4191—4203

Figure 2. Calycosin suppressed GBM progression and downregulated CXCL10 pathway in U87 and U251 cells. (A–C) The CCK8 experiment were performed in U87, U251 and HNA cells with the different concentrations of calycosin. (D, E) Cell colony formation in glioblastoma cells with CA treatments as indicated dose. (F–I) U87 and U251 cells were treated with the indicated dose of CA for 24 hours, and transwell assay was applied to examine the migration and invasion. (J) RT-PCR was applied to detect the mRNA level after indicated dose of CA. (K) U87 and U251 cells were performed with the different dose of CA for 24 hours and western bolt was applied to detect the protein expression levels of CXCL10 pathway. *P<0.05, **P<0.01, ***P<0.001, compared with control (0μM).