Research Paper Volume 16, Issue 4 pp 3973—3988
MicroRNA-320a enhances LRWD1 expression through the AGO2/FXR1-dependent pathway to affect cell behaviors and the oxidative stress response in human testicular embryonic carcinoma cells
Figure 8. Schematic diagram of miR-320 targeting human LRWD1 mRNA and influencing biological functions. miR-320a exerts a favorable regulatory effect on the expression of LRWD1 and NRF2 genes and proteins in response to oxidative stress. miR-320a potentially has a significant impact on cell behavior via interacting with the miRNP AGO2/FXR1 complex, eLF4E, and eEF2. These interactions may facilitate binding to LRWD1 and enhance mRNA transcriptional activity. Moreover, there is a positive correlation between miR-320a and LRWD1 and the proliferation of NT2D1 cells. However, they have a detrimental effect on cell migration and invasion. Therefore, under conditions of oxidative stress, elevated levels of miR-320a and LRWD1 promote the proliferation of NT2D1 testicular cancer cells.