Research Paper Volume 16, Issue 4 pp 3973—3988
MicroRNA-320a enhances LRWD1 expression through the AGO2/FXR1-dependent pathway to affect cell behaviors and the oxidative stress response in human testicular embryonic carcinoma cells
Figure 4. Overexpression of AGO2 and FXR1 enhanced the miR-320a-mediated increase in LRWD1 gene expression. (A) The enhancing effect of AGO2 and FXR1 overexpression in promoting LRWD1 expression was investigated by using the pMIR-LRWD1 3′UTR plasmid, miR-320a mimic and FXR1/AGO2 overexpression FLAG-tag plasmid. qRT-PCR results of LRWD1 gene expression regulated by overexpression of (B) FXR1 and (C) AGO2 under treatment with the miR-320a mimic or inhibitor. (D) The potential AGO2 and binding elements gene expression which directly increased miRNA target gene transcription. (*p < 0.05, **p < 0.01, ***p < 0.001 compared with the vector control or negative control; ###p < 0.001 compared with the miR-320a mimic-treated group).