Figure 1. The effect of miR-320a in regulating LRWD1 expression. (A) The predicted binding sequence of miR-320a on the LRWD1 3′UTR. (B) The effect of the deletion of LRWD1 on regulating endogenous miR-320a expression. (C) The regulatory effect of miR-320a on LRWD1 expression was demonstrated using transfecting cells with the miR-320a mimic or inhibitor by luciferase assay. (D) The effects of the miR-320a mimic and inhibitor on protein expression. (E) Quantitative analysis of LRWD1 protein expression regulated by the miR-320a mimic and inhibitor.