Research Paper Volume 16, Issue 4 pp 3934—3954
Functional enrichment analysis reveals the involvement of DARS2 in multiple biological pathways and its potential as a therapeutic target in esophageal carcinoma
Figure 6. Effects of DARS2 knockdown on ESCA glycolysis. (A, B) Knocking down DARS2 significantly reduces the uptake of 2-NBDG in ESCA cells. (C) DARS2 knockdown significantly reduces lactate production in ESCA cells. (D) GEPIA online database analysis revealed that DARS2 expression was significantly positively correlated with glycolysis signatures in ESCA. (E) The correlation between 11 glycolysis-related genes and DARS2 expression in the TCGA ESCA dataset. (F) Analyzing the expression differences of 11 glycolysis-related genes between the DARS2 high and low expression groups in the TCGA ESCA dataset. (G) The expression differences of 11 glycolysis-related genes between the experimental group and the control group were analyzed using qRT-PCR experiments. (H) The Venn diagram illustrates the genes that satisfy the aforementioned three conditions, including GPI and PFKL. *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.