Research Paper Volume 16, Issue 4 pp 3716—3733

Figure 3. RAB10 is a downstream target gene of miR-557, is overexpressed in HCC, and is related to poor prognosis. (A) Venn diagram of TargetScan, miWalk, miRDB, and miRTarBase. (B) Meta analysis of RAB10 expression from Oncomine database. (C) Predicted binding sites of 3′-UTR of RAB10 to miR-557. (D, E) The immunohistochemical images of relative RAB10 expression in HCC tissues and paired non-tumor tissues stem from the Human Protein Atlas database (Version 23.0, https://www.proteinatlas.org/ENSG00000084733-RAB10/pathology/liver+cancer#img). (F) The relationship between RAB10 expression and overall survival from the Human Protein Atlas database. (G, H) The relationship between the expression level of RAB10 and overall survival or disease-free survival from the Kaplan–Meier plotter online database. (I–L) The transfection efficiency of pmirGLO-NC, pmirGLO-RAB10 of MHCC-97H cells, and of siNC and siRAB10 of Huh7 cells. All blots were cut prior to hybridisation with antibodies during western blotting. (^***P<0.001, ^****P<0.0001).